microfluidics based immunoassay  (Protein Simple Inc)

Journal: The Lancet. Microbe

Article Title: Nasal host response-based screening for undiagnosed respiratory viruses: a pathogen surveillance and detection study

doi: 10.1016/S2666-5247(22)00296-8

Figure Lengend Snippet: Proteomic signature of top differentially expressed cytokines in patients with SARS-CoV-2 at the peak viral load versus end of infection and virus-negative health-care workers, using a multigroup comparison (A), and virus-positive patients and virus-negative health-care workers based on a two-group comparison (B). Cytokines were measured using a 71-plex immunoassay (Eve Technologies, Alberta, Canada). Heatmaps show cytokines clustered by coexpression and arranged by viral load (low Ct represents high viral load and high Ct represents low viral load). Z score represents SD from the mean. Peak of infection is defined as the longitudinal sample with highest viral load, and the end of infection is defined as the first sample with a Ct value higher than 30. (C) Receiver operating characteristic for CXCL10 concentrations based on values from an automated microfluidic assay, using a sample set in which the prevalence of viral respiratory infection is 34%. Cutoffs predicted to maximise sensitivity (blue) or specificity (yellow) for respiratory virus detection are shown. AUC=area under the curve.

Article Snippet: CXCL10 cutoffs for screening on a clinic-ready platform were defined using an automated microfluidics-based immunoassay (ProteinSimple, R&D Systems, Minneapolis, MN, USA) to measure CXCL10 concentrations in a previously described sample set., Metagenomic sequencing and phylogenetic analysis of SARS-CoV-2 and influenza C virus isolates was performed as previously described., The sample containing reads from influenza C virus was inoculated onto primary human nasal epithelial cells.

Techniques: Infection, Virus, Comparison